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and HRP conjugate are sequentially added to microwells pre-coated with a universal ciprofloxacin antigen
(5) Cells were counted using an automated cell counter and dead cells were excluded using trypan blue
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The standard curve is drawn with the standard concentration as the abscissa and the calibrated standard light absorption value as the ordinate
E-selectin has been shown to transduce an activating stimulus via the mitogen-activated protein kinase (MAPK) cascade into the endothelial cell during leukocyte adhesion
Alexa Fluor® 488 Mouse Anti-Human CD3d Antibody (S-R565) Size:100T and HRP conjugate are sequentiallyProduct Specification Host Mouse Antigen CD3d Synonyms T cell surface glycoprotein CD3 delta chain; T cell receptor T3 delta chain; T3D; CD3D Location Cell membrane Accession P04234 Clone Number S R565 Antibody Type Mouse mAb Isotype IgG2a Application FCM Reactivity Hu Positive Sample Human peripheral blood leukocytes Purification Protein A Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0.