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For best overall salt concentration should not exceed 50mM
A F Worrall，B A Connolly
combine the following reaction components in the order given:
The following amounts are for a single 20 μL reaction
Signaling & GTPase Biology
Pyrophosphatase Inorganic (yeast) Amount/Package:0.1kU / centrifuge tube For best overall salt concentrationProduct Information PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32 35kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates. Source: Recombinant E. coli Concentration: 0. 1U L Unit Definition: One unit is the amount of enzyme that will generate 1 mol of phosphate per minute from inorganic pyrophosphate
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